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Do you have any nitrate in your tap water? If not it has to be from something inside the tank or it's accessories that retained some pollutants.

During the month, did you have the usual ammonia spike, then nitrite spike? A lot of nitrate can be created just from the cycle.
 

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The 160ppm may just be from the cycle. I agree do 100% water change and hope for the best.
 
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