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100ppm nitrate is really high. I would be double-dosing Seachem Prime for your tank volume every two days. Now that is not a substitute for water changes! But, you've conquered one issue, and now get to battle the next one... (I am personally struggling with high nitrates and just purchased a de-nitrator, because I am sick of 3 water changes (60%-70%) per week to keep my nitrates below 20ppm. I can PM you if you want details.)italiiano said:I want to thank everybody for the help. I haven't lost any fish in the last two months, although one of the fish hasn't been looking good for the last month or so. But everybody is hanging in there. I've been using the buffer and have the pH around an 8.0. Even with weekly water changes though I'm struggling to get the Nitrates below 100. Any further suggestions? Thanks again guys.