If you are simply doing temporarily to move the tank away from the wall and then putting the water back, you can remove most of it. As long as they are covered by water, the fish will be fine.
When I moved my 55g across the room I went down to 1" of water above the substrate. Like the previous poster said as long as they are covered by the water and your filter media stays wet your fine.
Just be careful you don't pick up the tank off the stand with 1" water, substrate and rocks in there. With any weight in the tank, the frame must be supported by something absolutely level like the stand.
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