I think I would only put about 25 fish in the tank total, depending on the species. And if you cycle with ammonia you can probably put them all in at once.
Yes if one of the species is demasoni it would change upward. And if one of the species is auratus or crabro it would change downward.
You can cram more in. I had a really nice mbuna tank that wasw 72" for 2 years with 5 species and most of them were 1m:4f.
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