Appreciate the input from the forum, particularly DJ's advice on stocking. There are currently five Rusties, seven Perlmutt, seven Saulosi, and one Bristle nose pleco in 65 gallon. Started with five Perlmutt but four of five were males. Rehomed three males and purchased five juveniles, hoping for more females. Target is 1 male, 4 female ratio. Started with seven Rusties, rehomed two (gender still unknown on Rusties and no indication of a carrying female). Started with 12 Saulosi, rehomed four known males (might seem still be one male impersonating a female). Stripped one Perlmutt and one Saulosi and watching fry develop in separate 20G tank.