When I started fishless cycling, it took about a week and a half for the ammonia level to "zero out." I started out at ~5ppm, which I think was a little high, and although I used old filter material to seed the new filter, I didn't add anything like bio-spira. Remember that if you're using test strips or even the liquid reagent tests, the color chart isn't as accurate as a digital readout, so there could be change in ammonia levels that just aren't large enough to be picked up yet. Don't worry, patience works! 
