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In an existing matured tank, colonization will begin immediately. If you are looking at using this rock to "seed" another tank, then I'd leave the rock be for 3 weeks plus before using it to seed another tank.

Bacteria will begin to spread onto the rock but their doubling time depends on how much ammonia and O2 rushes on by that rock... so it becomes impossible to predict how long it takes. Filters are more predictable simply because we know that a max or ammonia and O2 rushes through the media.
 

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Discussion Starter · #5 ·
I see.

The reason I ask is because i just recently made a fry pile out of coral rocks from a recent trip to the beach. I then siliconed them to a flat piece of plexiglass and let it cure.

yesterday i placed it into my pond outside so all the excess salt could soak out, and plus i didnt want to directly place it into my cichlid tank in case the silicone hadnt cured properly, so i used the pond to test it out first. I figured after a couple of days i would take it out, rinse it well then place it into the main tank.
 
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