Thanks for the recommendations
@Auballagh!
With seeding the tank, yeah I did use three established MarinePure spheres from my current tank. Unfortunately I cannot remove too many from it since I feed the tank very heavily and so a lot of biological filtration is needed. Very quick calculation each sphere currently handles perhaps around 0.06ppm ammonia or so a day in a 10gal. Three in a 40gal should be able to handle 0.045ppm ammonia a day (probably less because only two is in the internal filter, one I placed near the sponge filter), so not much to begin with but should be okay to seed the tank. That's why I decided to get FritzZyme 7. The quality of bottled bac products vary greatly, but Fritz is one of those that is proven to work. I did not get the concentrated version, i.e. FritzZyme TurboStart 700 though, so it won't be a super quick cycle, but should still speed it up significantly. My aim is for the aquarium to handle 1ppm ammonia/day, which is normally plenty for a normal stocking even with decent feeding.
And yeah, definitely will keep an eye on the
R. lentiginosus in the aquarium. Worse case scenario we are likely to buy a house by September when an upgrade is entirely possible, so hopefully they don't grow too big by then lol.
Also I calculated, and assuming complete conversion of nitrogen in whatever the fish eats to ammonia, and assuming a 50% crude protein amount in said food, then they need to consume 1.4grams or so without using any nitrogen for growth to produce 1ppm ammonia. So should be fine for a while, just need to make sure I am not overfeeding, especially if they will also eat animals in the aquarium.