I've had to take my tank down to make a few repairs (leaky seals grrrrrrr) and I've only had one filter running on my holding tank. My other two filters has not been up and running, but I did leave water in them. Has all of the beneficial bacteria been lost? I've kept the substrate in a rubbermaid tub, with some water left in the bottom. When I get everything back up and running will I have to wait out before adding my fish back to the tank?