Remove One Test Strip from Bottle and Replace Cap Tightly. Hold Strip At End With No Pads. Drip Strip (All Pads) Into Aquarium Water and Remove Immediately. Do Not Shake Excess Water from Strip. Hold Strip Level to Allow Colors to Develop. At 30 Seconds, Compare Ph, Alkalinity, Hardness and Nitrite Pads to Color Charts Underneath Bottle Label. At 60 Seconds, Compare Nitrate Pad to Color Charts Provided. Record Results and Dispose of Test Strip. Keep Cap On Tight Between Uses. Store At Room Temperature. Do Not Expose to Moisture. Do Not Remove Drying Agent from Bottle As Test Results May Not Be Accurate Over Time.